4WZL
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type Lea (triglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.870, 58.830, 124.440 |
| Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
| Resolution | 42.467 - 1.700 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.19550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.590 | 1.610 | |
| High resolution limit [Å] | 1.570 | 7.030 | 1.570 |
| Rmerge | 0.052 | 0.025 | 0.487 |
| Rmeas | 0.063 | 0.030 | 0.591 |
| Total number of observations | 246067 | ||
| Number of reflections | 82519 | 977 | 5491 |
| <I/σ(I)> | 8.11 | 18.1 | 1.99 |
| Completeness [%] | 97.5 | 96.3 | 88.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate |
