4WZK
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type H2 (triglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.990, 58.810, 98.020 |
| Unit cell angles | 90.00, 108.11, 90.00 |
Refinement procedure
| Resolution | 29.655 - 1.490 |
| R-factor | 0.1406 |
| Rwork | 0.139 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oox |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.580 | 1.510 | |
| High resolution limit [Å] | 1.470 | 6.580 | 1.470 |
| Rmerge | 0.030 | 0.019 | 0.480 |
| Rmeas | 0.037 | 0.023 | 0.581 |
| Total number of observations | 302351 | ||
| Number of reflections | 102793 | 1178 | 7400 |
| <I/σ(I)> | 17.31 | 46.55 | 2.27 |
| Completeness [%] | 97.3 | 94.1 | 95.1 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate |
