4WZD
Complex of 70S ribosome with cognate tRNA-Tyr in the P-site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.700, 448.400, 616.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 153.540 - 3.100 |
| Rwork | 0.227 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVE 3TVF |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.200 | |
| High resolution limit [Å] | 3.100 | 100.000 | 3.100 |
| Rmerge | 0.216 | 0.018 | 2.958 |
| Rmeas | 0.224 | 0.020 | 3.072 |
| Total number of observations | 14622374 | ||
| Number of reflections | 1041276 | 14 | 93816 |
| <I/σ(I)> | 10.31 | 48.29 | 1.03 |
| Completeness [%] | 99.8 | 30.4 | 99.6 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
