4WZ0
Crystal structure of U-box 1 of LubX / LegU2 / Lpp2887 from Legionella pneumophila str. Paris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792063 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.334, 90.621, 40.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 1.954 |
| R-factor | 0.2378 |
| Rwork | 0.234 |
| R-free | 0.27810 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.168 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.096 | 0.418 |
| Number of reflections | 10142 | |
| <I/σ(I)> | 21.47 | 2.94 |
| Completeness [%] | 97.5 | 97.3 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | ~30 mg protein, 0.25 M ammonium sulfate, 0.1 M imidazole pH 7.8, 28% PEG 8K |
