4WYQ

Crystal structure of the Dicer-TRBP interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11111
Spacegroup name	F 41 3 2
Unit cell lengths	291.910, 291.910, 291.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 3.200
R-factor	0.23
Rwork	0.228
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.610
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	3.280
High resolution limit [Å]	3.200	3.200
Number of reflections	18119
<I/σ(I)>	18.55	1.7
Completeness [%]	99.9	99.8
Redundancy	13.2	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	2 uL of 7.3 g/L protein solution in buffer [0.01 M sodium acetate, 0.05 M sodium chloride, 0.001 M TCEP, and 5% (w/v) glycerol] was mixed with 1 uL [18% PEG 3350 (w/v), 0.26 M potassium sulfate, 0.001 M TCEP].