4WYP
The crystal structure of the A109G mutant of RNase A in complex with 5'AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.776, 51.725, 58.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.038 - 1.502 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wyn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.142 | 0.177 | 0.434 |
| Rmeas | 0.158 | 0.197 | 0.488 |
| Rpim | 0.068 | 0.085 | 0.220 |
| Total number of observations | 169142 | ||
| Number of reflections | 35457 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 94.6 | 96 | 87 |
| Redundancy | 4.8 | 4.7 | 4.5 |
| CC(1/2) | 0.965 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | MIB Buffer, PEG 6,000 |
