4WYK

Structure of the LRR and NTF2-like domains of NXF1 complexed with NXT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 1
Unit cell lengths	48.046, 83.972, 108.233
Unit cell angles	108.69, 99.76, 97.51

Refinement procedure

Resolution	46.411 - 3.400
R-factor	0.2158
Rwork	0.213
R-free	0.26560
RMSD bond length	0.004
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.411
High resolution limit [Å]	3.400
Number of reflections	19892
<I/σ(I)>	6.5
Completeness [%]	93.5
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	9 % PEG 6000, 1 M LiCl, 0.1 M sodium citrate buffer pH 5.1 See publication for full details.