4WY4

Crystal structure of autophagic SNARE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	82.850, 35.030, 104.200
Unit cell angles	90.00, 94.09, 90.00

Refinement procedure

Resolution	51.967 - 1.400
R-factor	0.1383
Rwork	0.136
R-free	0.17680
RMSD bond length	0.011
RMSD bond angle	1.174
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.970		1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.063	0.052	0.671
Rmeas	0.068	0.056	0.726
Total number of observations	385970
Number of reflections	58501	733	4231
<I/σ(I)>	14.32	28.91	2.51
Completeness [%]	98.7	99.2	98.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M BICINE pH 8.5, 55% (v/v) MPD