4WXO
SadC (300-487) from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.419, 116.449, 88.216 |
| Unit cell angles | 90.00, 110.19, 90.00 |
Refinement procedure
| Resolution | 39.463 - 2.805 |
| R-factor | 0.2027 |
| Rwork | 0.200 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wxw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.215 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.388 |
| Number of reflections | 18174 | |
| <I/σ(I)> | 46.74 | 7.99 |
| Completeness [%] | 98.8 | 98.6 |
| Redundancy | 7.3 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293.15 | 0.1 M Tris-HCl, 1.5 M NH4SO4, 12% glycerol |
