4WXE
CRYSTAL STRUCTURE OF A LACI REGULATOR FROM LACTOBACILLUS CASEI (LSEI_2103, TARGET EFI-512911) WITH BOUND TRIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 2 |
Unit cell lengths | 108.047, 108.047, 121.477 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.473 - 1.500 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.16810 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.262 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.473 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.123 | 0.952 |
Rpim | 0.027 | 0.207 |
Total number of observations | 964401 | 46843 |
Number of reflections | 43692 | |
<I/σ(I)> | 21.5 | 4.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 22.1 | 21.9 |
CC(1/2) | 0.999 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Protein (30.7 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT); Reservoir ((MCSG4 F4), 0.2 M Magnesium Chloride, 0.1 M Tris pH 8.5, 16%(w/v) PEG 4000, 0.2M Lithium Chloride, 20 %(w/v) PEG 3350); Cryoprotection (80% Reservoir + 20% Ethylene Glycol |