4WX0
UndA complexed with beta-hydroxydodecanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 67.410, 73.990, 142.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.750 - 1.700 |
| R-factor | 0.13907 |
| Rwork | 0.137 |
| R-free | 0.18454 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.378 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.750 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 75678 | |
| <I/σ(I)> | 14.5 | 1.1 |
| Completeness [%] | 95.8 | 70.9 |
| Redundancy | 8.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M MES, 1.8 M ammonium sulfate, 0.2 mM iron(II) ammonium sulfate, pH 6.5-7.0 |
