4WWV
Aminopeptidase APDkam598 from the archaeon Desulfurococcus kamchatkensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 234.323, 234.323, 234.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.831 - 3.006 |
| R-factor | 0.2074 |
| Rwork | 0.204 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xfo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | |
| Number of reflections | 21317 | |
| <I/σ(I)> | 19.4 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.5 | 293 | The reservoir solution contained 0.1 M HEPES, pH 7.0, 0.8 M ammonium sulfate, and glycerol. The crystals were grown over a period of several months. |
