4WWS
Structure of Chlorite dismutase-like Protein from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.000, 128.320, 78.150 |
| Unit cell angles | 90.00, 105.91, 90.00 |
Refinement procedure
| Resolution | 48.758 - 2.000 |
| R-factor | 0.2015 |
| Rwork | 0.198 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nn1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.591 |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.196 | 0.939 |
| Number of reflections | 99373 | |
| <I/σ(I)> | 6.77 | 1.59 |
| Completeness [%] | 99.8 | 99.99 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 1.1 M Na K tartrate, 8% glycerol, 0.1 M TrisHCl, 1/10 Silver Bullets 52: Protamine Sulfate |
