4WW4
Double-heterohexameric rings of full-length Rvb1(ADP)/Rvb2(ADP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97239 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 206.848, 206.848, 137.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.160 - 2.940 |
| R-factor | 0.1989 |
| Rwork | 0.198 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wvy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.161 | 64.161 | 3.100 |
| High resolution limit [Å] | 2.940 | 9.300 | 2.940 |
| Rmerge | 0.038 | 0.690 | |
| Rmeas | 0.116 | ||
| Rpim | 0.052 | 0.019 | 0.432 |
| Total number of observations | 103751 | 3977 | 10883 |
| Number of reflections | 23417 | ||
| <I/σ(I)> | 8.3 | 21.9 | 1.7 |
| Completeness [%] | 97.4 | 95.5 | 96.9 |
| Redundancy | 4.4 | 5.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1 M sodium malonate, ADP-BeF3 |
