4WW4

Double-heterohexameric rings of full-length Rvb1(ADP)/Rvb2(ADP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97239
Spacegroup name	H 3 2
Unit cell lengths	206.848, 206.848, 137.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.160 - 2.940
R-factor	0.1989
Rwork	0.198
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wvy
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.161	64.161	3.100
High resolution limit [Å]	2.940	9.300	2.940
Rmerge		0.038	0.690
Rmeas	0.116
Rpim	0.052	0.019	0.432
Total number of observations	103751	3977	10883
Number of reflections	23417
<I/σ(I)>	8.3	21.9	1.7
Completeness [%]	97.4	95.5	96.9
Redundancy	4.4	5.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1 M sodium malonate, ADP-BeF3