4WW3
Crystal structure of the lumi intermediate of squid rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 62 |
| Unit cell lengths | 122.390, 122.390, 158.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.080 - 2.800 |
| R-factor | 0.29 |
| Rwork | 0.290 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z73 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | CNS (1.2) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.848 | 52.848 | 2.950 |
| High resolution limit [Å] | 2.799 | 8.850 | 2.799 |
| Rmerge | 0.070 | 0.494 | |
| Rpim | 0.033 | 0.252 | |
| Total number of observations | 3456 | 17666 | |
| Number of reflections | 28450 | ||
| <I/σ(I)> | 7.1 | 15.8 | 2.1 |
| Completeness [%] | 85.9 | 71.2 | 91.4 |
| Redundancy | 4.2 | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277 | 3.0M ammonium sulfate, 20mM MES, 50mM EDTA, 10mM beta-mercaptoethanol |
