4WVW
Chicken Galectin-8 N-terminal domain complexed with 3'-sialyl-lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 56.459, 56.459, 198.987 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.480 - 1.470 |
| R-factor | 0.1773 |
| Rwork | 0.176 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yv8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.130 | 1.807 |
| Number of reflections | 33211 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.5 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 30% w/v PEG 4000, 100 Mm Tris-HCl pH 8.5, 200 mM Sodium Acetate |
