4WV4
Heterodimer of TAF8/TAF10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.320, 51.320, 144.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.444 - 1.909 |
| R-factor | 0.2064 |
| Rwork | 0.205 |
| R-free | 0.23730 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.681 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.444 | 1.980 |
| High resolution limit [Å] | 1.909 | 1.910 |
| Rmerge | 0.029 | 0.880 |
| Number of reflections | 16793 | |
| <I/σ(I)> | 23.4 | 1.68 |
| Completeness [%] | 93.9 | 58.53 |
| Redundancy | 4.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 281.15 | sodium/potassium phosphate |
