4WUL

Crystal structure of E. faecalis DNA binding domain LiaRD191N complexed with 26bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 62
Unit cell lengths	113.585, 113.585, 48.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.445 - 2.400
R-factor	0.224
Rwork	0.218
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4WTO
RMSD bond length	0.004
RMSD bond angle	0.686
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.400	2.400
Rmerge	0.101	0.733
<I/σ(I)>	33	9.333
Completeness [%]	100.0	100
Redundancy	22.5	22.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	283	0.08 M KCl, 0.02 M BaCl2, 0.04 M Sodium cacodylate (pH 6.0), 43% MPD, 0.012 M Spermine tetrachloride, 0.01 M Proline, 0.01 M Strontium chloride