4WUK
Crystal structure of apo CH65 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.095, 67.024, 130.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.763 - 1.700 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.20730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sm5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.209 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.820 | |
| Number of reflections | 55829 | |
| <I/σ(I)> | 14.7 | 2.5 |
| Completeness [%] | 99.6 | 93.3 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293 | 1.8 M ammonium sulfate, 0.1 M CHES |
