4WUH

Crystal structure of E. faecalis DNA binding domain LiaR wild type complexed with 22bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	38.332, 77.932, 104.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.746 - 2.294
R-factor	0.219
Rwork	0.215
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.638
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.107	0.431
<I/σ(I)>	25	7.176
Completeness [%]	100.0	100
Redundancy	14.1	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	283	0.2 M Magnesium formate dihydrate, 20%w/v PEG 3,350, 0.012 M Spermine tetrachloride.