4WU7

HLA-A24 in complex with HIV-1 Nef134-8(2F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.714, 87.020, 152.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.297
R-factor	0.2248
Rwork	0.222
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxn
RMSD bond length	0.007
RMSD bond angle	1.114
Data reduction software	SCALEPACK
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.297
Number of reflections	37537
<I/σ(I)>	14.06
Completeness [%]	93.0
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM sodium nitrate, 20%PEG3350