4WTZ

Human CEACAM6-CEACAM8 N-domain heterodimer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	109.146, 113.582, 123.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.490 - 2.520
R-factor	0.2064
Rwork	0.205
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4whc
RMSD bond length	0.017
RMSD bond angle	2.046
Data reduction software	d*TREK
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	83.490
High resolution limit [Å]	2.520
Number of reflections	52367
<I/σ(I)>	17.8
Completeness [%]	100.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 2000 MME, NiSO4, Tris-HCl