4WTY
Structure of the PTP-like myo-inositol phosphatase from Selenomonas ruminantium in complex with myo-inositol-(1,3,4,5)-tetrakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.830, 138.150, 80.580 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 43.060 - 2.100 |
| R-factor | 0.15724 |
| Rwork | 0.157 |
| R-free | 0.17538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.067 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.060 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.135 | 0.481 |
| Number of reflections | 56995 | |
| <I/σ(I)> | 5.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 8000, sodium chloride, sodium acetate, beta-mercapto ethanol, |
