4WTH
Ataxin-3 Carboxy Terminal Region - Crystal C2 (triclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.096, 59.786, 77.790 |
| Unit cell angles | 90.00, 89.99, 87.50 |
Refinement procedure
| Resolution | 77.790 - 2.250 |
| R-factor | 0.20424 |
| Rwork | 0.202 |
| R-free | 0.24975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.743 | 2.360 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.047 | |
| Number of reflections | 39115 | |
| <I/σ(I)> | 15.7 | 1.2 |
| Completeness [%] | 94.0 | 76 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% PEG5000 MME, 0.9 M sodium acetate, 0.06 M imidazole, pH 8.0, 0.1 M zinc acetate |
