4WT3
The N-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 29.769, 29.769, 457.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.706 - 1.954 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.24560 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.090 | 45.708 | 2.060 |
| High resolution limit [Å] | 1.950 | 6.180 | 1.950 |
| Rmerge | 0.022 | 1.067 | |
| Rmeas | 0.071 | ||
| Rpim | 0.019 | 0.007 | 0.308 |
| Total number of observations | 222430 | 7309 | 28898 |
| Number of reflections | 16733 | ||
| <I/σ(I)> | 20.2 | 67 | 2.1 |
| Completeness [%] | 100.0 | 99.7 | 99.8 |
| Redundancy | 13.3 | 10.3 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES-NaOH pH 6.0 and 26% (w/v) PEG-6000 |
