4WSX
The crystal structure of hemagglutinin from A/Jiangxi-Donghu/346/2013 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 217.395, 217.612, 146.266 |
Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.700 |
R-factor | 0.24441 |
Rwork | 0.243 |
R-free | 0.26578 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.510 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 185693 |
<I/σ(I)> | 18.2 |
Completeness [%] | 100.0 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2M potassium sodium tartrate and 20% (w/v) PEG 3350 |