4WSU
The crystal structure of hemagglutinin from A/Taiwan/1/2013 in complex with 3'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.978, 100.080, 135.239 |
| Unit cell angles | 90.00, 126.04, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.700 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 54578 |
| <I/σ(I)> | 20.6 |
| Completeness [%] | 98.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 0.01M nickel chloride, 0.1 M Tris-HCl pH 8.5 and 20% (w/v) PEG 2000MME |
