4WSG
Crystal Structure of Soluble WR PIV5 F-GCNt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.07808 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.528, 155.523, 157.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.853 - 3.000 |
| R-factor | 0.2329 |
| Rwork | 0.230 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b9b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.317 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.450 | 3.000 |
| Rmerge | 0.122 | 0.044 | 0.610 |
| Total number of observations | 209764 | ||
| Number of reflections | 41057 | ||
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 98.4 | 100 | 90.8 |
| Redundancy | 5.1 | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.09 M Carboxylic acids (Na-Formate, NH4-Acetate, Na3-Citrate, NaK-Tartrate (racemic), Na-Oxamate); 0.09 M Tris/Bicine; and 27% wt/vol Glycerol/PEG 4000 |
