4WSF

Falafel EVH1 domain bound to CENP-C FIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 61
Unit cell lengths	67.755, 67.755, 53.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.678 - 1.501
R-factor	0.1364
Rwork	0.134
R-free	0.17640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xod 1rrp
RMSD bond length	0.009
RMSD bond angle	1.174
Data reduction software	XDS (0.2.17)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.680	58.680	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.079	0.042	0.970
Rpim	0.032	0.017	0.394
Total number of observations	150514	1013	7243
Number of reflections	22313
<I/σ(I)>	11.7	38.6	1.7
Completeness [%]	99.7	99.9	96.5
Redundancy	6.7	6.7	6.8
CC(1/2)	0.999	0.999	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium Sulphate