4WSC
Crystal structure of a GroELK105A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.640, 259.390, 143.310 |
| Unit cell angles | 90.00, 105.12, 90.00 |
Refinement procedure
| Resolution | 80.523 - 3.040 |
| R-factor | 0.1908 |
| Rwork | 0.190 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xck |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.150 | 3.090 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.190 | 0.758 |
| Number of reflections | 180177 | |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 98.5 | 95.2 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, HEPES |
