4WRP
The C-terminal domain of gene product lpg0944 from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.352, 60.714, 57.523 |
| Unit cell angles | 90.00, 104.61, 90.00 |
Refinement procedure
| Resolution | 38.500 - 1.900 |
| R-factor | 0.1645 |
| Rwork | 0.162 |
| R-free | 0.20540 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.066 | 0.060 | 0.402 |
| Total number of observations | 99589 | ||
| Number of reflections | 22416 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 97.9 | 92.8 | 95.5 |
| Redundancy | 4.4 | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291 | 2.8M NaCl 0.1M Tris-HCl pH8.5 2% MPD |
