4WRI

Crystal structure of okadaic acid binding protein 2.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	42.970, 64.620, 145.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.743 - 1.400
R-factor	0.1752
Rwork	0.174
R-free	0.20500
RMSD bond length	0.008
RMSD bond angle	1.186
Data reduction software	XDS
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.743		1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.036	0.021	0.350
Rmeas	0.039	0.024	0.388
Total number of observations	277458
Number of reflections	39985	387	2892
<I/σ(I)>	28.58	52.83	4.64
Completeness [%]	99.2	74.9	97.2
Redundancy	6.94		5.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.2 M NaOAc, 0.1 M Tris-HCl(pH 8.0), 30-40% PEG 4000