4WRG
1.9 angstrom structure of EGFR kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 143.182, 143.182, 143.182 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.900 |
| R-factor | 0.19809 |
| Rwork | 0.197 |
| R-free | 0.21179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2itx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.506 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 41627 | |
| <I/σ(I)> | 14 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 38.5 | 36.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M MES, 1 M sodium citrate |
