4WRD
Crystal structure of Staphylcoccal nulease variant Delta+PHS V66E L125E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-08-04 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.140, 60.315, 38.364 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 38.270 - 1.650 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.620 |
| Data reduction software | SAINT (2013/3 for Windows) |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.270 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.010 | 0.140 |
| Total number of observations | 477720 | |
| Number of reflections | 17155 | |
| <I/σ(I)> | 142.68 | 6.63 |
| Completeness [%] | 99.9 | 97.6 |
| Redundancy | 27.81 | 8.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25mM potassium phosphate, calcium chloride, pdTp |
