4WQS

Thermus thermophilus RNA polymerase backtracked complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-14
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	155.983, 155.983, 495.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.150 - 4.306
R-factor	0.2825
Rwork	0.281
R-free	0.31140
RMSD bond length	0.010
RMSD bond angle	1.741
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.3)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.450
High resolution limit [Å]	4.300	4.300
Rmerge		0.811
<I/σ(I)>	11.9	2.7
Completeness [%]	99.7	99.9
Redundancy	9.1	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG-8000, calcium chloride, 1,6-hexanediol, Tris