4WQR

Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the first position in the A-site.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	210.100, 448.800, 621.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.190 - 3.150
Rwork	0.189
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3TVE 3TVF
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	250.000		3.250
High resolution limit [Å]	3.150	150.000	3.150
Rmerge	0.371	0.207	4.755
Rmeas	0.373	0.219	4.798
Total number of observations	83596048
Number of reflections	1000693	1	88990
<I/σ(I)>	15.45	19.31	1.19
Completeness [%]	99.9	6.7	99.9
Redundancy	83.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-acetate, KSCN, PEG 550MME, PEG 20K