4WQN

Crystal structure of N6-methyladenosine RNA reader YTHDF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	76.689, 76.689, 131.241
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.344 - 2.121
R-factor	0.1878
Rwork	0.186
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yu6
RMSD bond length	0.008
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.200
High resolution limit [Å]	2.120	4.570	2.120
Rmerge	0.113	0.059	0.898
Rmeas	0.119	0.062	0.949
Rpim	0.037	0.019	0.302
Total number of observations	259897
Number of reflections	24685
<I/σ(I)>	9
Completeness [%]	100.0	99.9	100
Redundancy	10.5	10.4	9.5
CC(1/2)		0.998	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	277	0.1M phosphate-citric pH 4.3, 0.2M Lithium Sulfate, 10% PEG8000