4WPZ

Crystal structure of cytochrome P450 CYP107W1 from Streptomyces avermitilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.96418
Spacegroup name	P 43 21 2
Unit cell lengths	114.877, 114.877, 79.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.540 - 2.100
R-factor	0.1815
Rwork	0.178
R-free	0.24020
RMSD bond length	0.019
RMSD bond angle	2.194
Data reduction software	SCALEPACK
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	31242
<I/σ(I)>	40
Completeness [%]	99.1
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	KBr, PEG 2000