4WPK
Crystal structure of Mycobacterium tuberculosis uracil-DNA glycosylase, Form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.72932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.919, 63.473, 45.186 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 23.740 - 0.980 |
| R-factor | 0.13746 |
| Rwork | 0.137 |
| R-free | 0.15416 |
| Starting model (for MR) | 3a7n |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.912 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.740 | 1.030 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Number of reflections | 115505 | |
| <I/σ(I)> | 9.4 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 1.6 M Sodium citrate tribasic dihydrate |
