4WPH
Crystal structure of USP7 ubiquitin-like domains in compact conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.336, 92.336, 190.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.920 |
| R-factor | 0.2342 |
| Rwork | 0.233 |
| R-free | 0.26190 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.290 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 3.020 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 21012 | |
| <I/σ(I)> | 22.19 | 4 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 1.86 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 3000, sodium citrate, L-proline |
