4WPA
Crystal structure of Adenylyl cyclase Ma1120 from Mycobacterium Avium bound to Pyrophosphate and Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.720, 55.720, 55.330 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 37.985 - 1.700 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wp8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.054 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.636 |
| Number of reflections | 33241 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | 0.1M HEPES, 30%(w/v) PEG 1000 |
