4WP4

Hev b 6.02 (hevein) extracted from surgical gloves

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-01-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	37.330, 37.330, 48.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.947 - 1.430
R-factor	0.1529
Rwork	0.151
R-free	0.16640
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.457
Refinement software	PHENIX ((phenix.refine: dev_1810))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.950	1.480
High resolution limit [Å]	1.430	1.430
Rmerge	0.058	0.186
Number of reflections	13802
<I/σ(I)>	64.6	24.4
Completeness [%]	99.0	96.7
Redundancy	11.8	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1M sodium citrate, 2M ammonium sulfate