4WP4
Hev b 6.02 (hevein) extracted from surgical gloves
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-01-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 37.330, 37.330, 48.777 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.947 - 1.430 |
R-factor | 0.1529 |
Rwork | 0.151 |
R-free | 0.16640 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.457 |
Refinement software | PHENIX ((phenix.refine: dev_1810)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.950 | 1.480 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.058 | 0.186 |
Number of reflections | 13802 | |
<I/σ(I)> | 64.6 | 24.4 |
Completeness [%] | 99.0 | 96.7 |
Redundancy | 11.8 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1M sodium citrate, 2M ammonium sulfate |