4WNZ

Crystal structure of Pyrococcus furiosus Cmr4 (Cas7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 41
Unit cell lengths	63.997, 63.997, 195.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.817 - 2.800
R-factor	0.2002
Rwork	0.197
R-free	0.22810
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.660
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.084	0.476
Number of reflections	19448
<I/σ(I)>	13.7	2.4
Completeness [%]	98.8	93.1
Redundancy	3.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM sodium citrate, 7% w/v PEG 3350