4WNX
Netrin 4 lacking the C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.755, 75.049, 74.672 |
| Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
| Resolution | 33.490 - 2.723 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aqs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.490 |
| High resolution limit [Å] | 2.720 |
| Number of reflections | 14673 |
| <I/σ(I)> | 5 |
| Completeness [%] | 91.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG3350, calcium acetate, sodium cacodylate, NDSB-256 |
