4WNQ
THE MOLECULAR BASES OF DELTA/ALPHA-BETA T-CELL MEDIATED ANTIGEN RECOGNITION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.257, 47.235, 109.290 |
| Unit cell angles | 88.32, 79.78, 89.93 |
Refinement procedure
| Resolution | 47.210 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.750 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 69885 | |
| <I/σ(I)> | 14.5 | 3.4 |
| Completeness [%] | 96.3 | 96.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 27% PEG 1500, 0.1M Sodium Malonate-Imidazole-Borate (2:3:3 molar ratio) |
