4WNL

The X-ray structure of a RNA-binding protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	C 1 2 1
Unit cell lengths	175.620, 64.670, 116.370
Unit cell angles	90.00, 117.94, 90.00

Refinement procedure

Resolution	59.692 - 2.800
R-factor	0.2143
Rwork	0.212
R-free	0.25430
RMSD bond length	0.002
RMSD bond angle	0.579
Refinement software	PHENIX ((phenix.refine: 1.9_1678))

Data quality characteristics

	Overall
Low resolution limit [Å]	83.540
High resolution limit [Å]	2.800
Number of reflections	28752
<I/σ(I)>	12.3
Completeness [%]	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	289.15	Tri-sodium citrate, pH 6.4, 13% (w/v) PEG 4K, 100mM (NH4)2SO4 and 3% v/v 2-Propanol