4WN4
Crystal structure of designed cPPR-polyA protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.07253 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 204.720, 204.720, 204.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.791 - 3.850 |
| R-factor | 0.1836 |
| Rwork | 0.179 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pjq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.950 |
| High resolution limit [Å] | 3.850 | 3.850 |
| Rmerge | 0.100 | 1.960 |
| Number of reflections | 6865 | |
| <I/σ(I)> | 23 | 2.18 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20 | 19.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2 M magnesium chloride, 0.1 M Tris pH 7.0, 10% w/v PEG 8000 |
