4WN3
Crystal structure of Saccharomyces cerevisiae OMP synthase in complex with PRP(NH)P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.010, 62.010, 132.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.170 - 1.800 |
| R-factor | 0.20253 |
| Rwork | 0.201 |
| R-free | 0.23763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wml |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.140 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24622 | |
| <I/σ(I)> | 28.36 | 1.6 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 14.2 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Na HEPES pH 7.5, 10 % isopropanol, 20 % PEG 4000 |
