4WMJ
Colias eurytheme Phosphoglucose isomerase. Homodimer from 4-5(18) genotype.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2011-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.287, 115.495, 135.079 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 41.190 - 1.500 |
| R-factor | 0.1849 |
| Rwork | 0.183 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u0e |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.190 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 270174 |
| <I/σ(I)> | 8.99 |
| Completeness [%] | 77.7 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 0.1M lithium sulfate, 22% PEG 4000, 0.2M Tris-Cl pH 7.4. |
