4WM3

High pressure protein crystallography of hen egg white lysozyme at 710 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2012-12-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.71
Spacegroup name	P 43 21 2
Unit cell lengths	76.661, 76.661, 37.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.910 - 1.550
R-factor	0.18517
Rwork	0.181
R-free	0.25912
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.011
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Number of reflections	16944
<I/σ(I)>	48.9
Completeness [%]	99.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride